DENATURING GRADIENT GEL ELECTROPHORESIS OF NITRIFYING MICROBES ASSOCIATED WITH TROPICAL SPONGES

Autori

  • MARIA CRISTINA DIAZ

Abstract

This study aims to explore the presence and phylogenetic diversity of ammonium oxidizing bacteria (AOB) among abundant sponges in mangrove communities. Using nested PCR amplifications, DGGE electrophoresis, and direct sequencing of DGGE bands, the presence of AOB sequences from five of the six common sponge species was revealed (Haliclona implexiformis, Lissodendoryx isodictyalis, Tedania ignis, Geodia papyracea, Chondrilla nucula). The phylotypes encountered belonged to the Nitrosomonas eutropha/europa lineage (cluster seven) of the ?- Proteobacteria. This indicates an affinity of sponge-associated AOB with those that prevail on marine-derived particles. This study suggests a widespread occurrence of AOB among Caribbean sponges whose role in the nutrient cycling on mangrove ecosystems remains to be evaluated.

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Pubblicato

2018-05-22

Fascicolo

V. 68 (2004): Sponge Science in the new Millennium “Papers contributed to the VI International Sponge Conference“ Rapallo (Italy) 29th September - 5th October 2002

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