A MOLECULAR SYSTEMATIC SURVEY OF SPONGE-DERIVED MARINE MICROBES

Autori

  • JOSE V. LOPEZ

Abstract

A systematic survey is being conducted to determine the microbial diversity held within the Harbor Branch Oceanographic Marine Microbial Culture Collection (HBMMCC). The collection consists of an estimated 16,500 marine microorganisms, with 11,000 from > 150 ft seawater. To date, around 1,000 microbes have been inventoried using the DNA fingerprinting technique RFLP (restriction fragment length polymorphism) on the small subunit (SSU) 16S (eubacterial) and 18S (fungal) rRNA gene. RFLP patterns obtained from restriction digests with the enzymes RsaI and HaeIII are used to “infer” taxonomic similarity of isolates. About 750 base pairs (bp) of the 5' portion of the 16S or 18S SSU rRNA gene was sequenced from representative isolates for more definitive phylogenetic analysis. Preliminary results show that the HBMMCC contains isolates from at least 6 major bacterial clades (Proteobacteria (Alpha, Beta, Gamma), CFB (Cytophaga, Flavobacteria, and Bacteroides), Gram+ High GC Content, Gram+ Low GC Content) with the possible discovery of novel microbial taxa.

Downloads

Pubblicato

2018-05-23

Fascicolo

V. 68 (2004): Sponge Science in the new Millennium “Papers contributed to the VI International Sponge Conference“ Rapallo (Italy) 29th September - 5th October 2002

Sezione

Articoli

Licenza

Authors who publish in this journal accept the following conditions:

  • Authors mantain the rights to their work and give the journal the right of first publication, simoultaneously licensed under a Creative Common International License 4.0: Attribution - Noncommercial - Share-Alike, which allows to share the work declaring the intellectual paternity and the first publication in this journal
  • Authors can subscribe othe non exclusive license agreements to distribute the published version of their work (eg deposit it in an institutional archive or publish it in a monograph) only if they declare that the first publication was in this journal.
  • The authors can spread their work online (eg in institutional repositories or on their own website) at the end of submission process (post print version).